Sunday, 11 October 2009
Delegates are also invited to register to attend four of short courses on Sunday, 11th October at the Hilton Walt Disney Hotel. There are two full day courses and two half day courses.
As details of courses are finalised, the timings and programmes will be posted on the conference website.
Short Course 1
Sample preparation for LC-MS/MS analysis of small molecules in biofluids
Instructors:
Prof. Dr. Dr. Karl-Siegfried Boos and Dr Rosa Morello
University of Munich, Germany
Short Course 1 is a full day course. The course fee includes a light lunch, two refreshment breaks and a copy of the course notes. The timings of the day will be confirmed in due course.
Short Course 2 (half day)
MS based Metabolomics - cancelled, places still availabe on short courses 1 and 4
Short Course 3 (half day)
SPME – Solide Phase Microextraction - cancelled, places still availabe on short courses 1 and 4
Short Course 4
MS of peptides and proteins
Instructor:
Prof Arthur Moseley
Duke University, USA
Short Course 4 is a full day course. The course fee includes a light lunch, two refreshment breaks and a copy of the course notes. The timings of the day will be confirmed in due course.
Short Course 1
Sample preparation for LC-MS/MS analysis of small molecules in biofluids
Instructors:
Prof. Dr. Dr. Karl-Siegfried Boos and Dr Rosa Morello
University of Munich, Germany
This is a full day course. The course fee includes a light lunch, two refreshment breaks and a copy of the course notes. The timings of the day will be confirmed in due course.
Cost: US$300
Course contents:
This short course is dedicated to technicians and chemists responsible for method development, analytical chemists, laboratory supervisors and scientists in the biochemical, pharmaceutical, clinical and environmental setting but also in other fields. It will cover instrumentation, operational procedures and practical guidelines for integration of solid-phase extraction (SPE) into LC-systems coupled to tandem mass spectrometry. A focus of this course will also be the principles and application of multidimensional SPE. Properties and performance of tailor-made SPE packing materials (e.g. restricted access materials, RAM, molecularly imprinted polymers, MIP, mixed-mode materials such as Oasis, etc.) as well as high throughput and miniaturization of SPE-LC will be discussed in detail. Besides direct injection and on-line SPE of urine and plasma samples, the direct injection and in-line processing of whole blood will be addressed. A general introduction to the understanding, monitoring and elimination of ion suppression / matrix effects in bioanalytical LC-MS/MS will be given. Special attention will be given to Phase Optimized Liquid Chromatography (POPLC) and its application in SPE-LC-MS/MS. Finally, a broad spectrum of applications in the fields of therapeutic drug monitoring, biological monitoring, environmental – and clinical-chemical analysis will be presented.
Instructors:
Prof. Dr. Dr. Karl-Siegfried Boos
University of Munich, Germany
1968 - 1977 Technical and Medical University, Hannover, Germany; 1974 Graduation in Biochemistry; 1977 Academic degree: Dr. rer. nat; 1977 - 1978 Department of Biochemistry, Washington State University, USA; 1979 - 1990 Laboratory of Biological Chemistry, University of Paderborn; 1987 Professor of Biological Chemistry. Since 1990 Head of the Laboratory of BioSeparation at the Institute of Clinical Chemistry, Medical Center of the Ludwig-Maximilians University (LMU), Munich; 1994 Professor of Experimental Clinical Chemistry and academic degree Dr. rer. biol. hum. Over 100 original publications in the field of Biochemistry, Analytical Chemistry and Medicine. Since 2000 co-editor of the journal “Chromatographia”.
Dr. Rosa Morello
University of Munich, Germany
1995 - 2000 Student of Pharmacy at the Universities of Würzburg and Munich; 2000 -2001 Practical year and Approbation as Pharmacist; 2001 - 2004 Ph.D. student of Prof. Dr. Dr. Karl-Siegfried Boos, Laboratory of BioSeparation, Medical Center of the Ludwig-Maximilians University (LMU), Munich; 2007 Doctoral degree Dr. rer. biol. hum. from the Medical Faculty of the LMU; 2004-to date Senior research scientist at the same laboratory. Expert for preanalytics and integrated clean-up of complex (bio)fluids as well as their on-line analysis with high performance separation and detection platforms (e.g. SPE-LC-MS/MS). Presentation of numerous basic and advanced courses on this topic.
Short Course 2 (half day)
MS based Metabolomics
Instructor:
Prof Gary Siuzdak
Scripps Research Institute, USA
This is a half day course to be run during the morning of Sunday, 11th October. The course fee includes a refreshment break and copies of the course notes. The timings of the afternoon will be confirmed in due course.
Cost: US$150
This course is designed for the scientist who wants to learn about mass spectrometry-based metabolomics. The course covers a brief history, sample preparation, choice of ionization methods and mass spectrometer, as well as data preparation and analysis, structural characterization and biomarker validation. Discussions will include the optimization of sample preparation for metabolite extraction and protein removal, as well as choosing the most appropriate ionization method and mass spectrometer. The course also highlights the following topics: metabolite imaging, metabolite structural characterization using MS/MS for fragmentation data, and accurate mass measurements usingMS/MS fragmentation data and accurate mass measurements using FTMS. Further characterization approaches will be discussed, as well as biomarker validation.
Instructor:
Gary Siuzdak
Scripps Research Institute, USA
Dr. Siuzdak is Senior Director of the Scripps Center for Mass Spectrometry and Professor of Molecular Biology at The Scripps Research Institute in La Jolla, California (http://masspec.scripps.edu/). He is also Faculty Guest at Lawrence Berkeley National Laboratory and Vice President of the American Society for Mass Spectrometry. His research includes developing novel approaches to metabolomics and proteomics, the development of nanostructure-initiated desorption/ionization, intact viral analysis, preparative mass spectrometry, and mass-based inhibitor-enzyme screening. He has over 140 peer-reviewed publications and three books, the latest being The Expanding Role of Mass Spectrometry in Biotechnology, 2nd Edition 2006.
Short Course 3 (half day)
SPME
Instructor:
Prof Janusz Pawliszyn
University of Waterloo, Canada
This is a half day course to be run during the afternoon of Sunday, 11th October. The course fee includes a refreshment break. The official course textbook and cd can also be ordered at discounted price of $50.00. The timings of the afternoon will be confirmed in due course.
Cost: US$150
plus $50 for the official textbook for the course: Handbook of Solid Phase Microextraction, Pawliszyn, J. (editor).
SPME - SOLID-PHASE MICROEXTRACTION FOR PHARMACEUTICAL APPLICATIONS
Dajana Vuckovic and Janusz Pawliszyn
University of Waterloo, Canada
Solid-phase microextraction (SPME) is a simple, fast, sensitive, and environmentally-friendly equilibrium-based sample preparation technique that allows the integration of sampling and sample preparation steps. This short course will describe the fundamental thermodynamic and kinetic principles of SPME followed by a discussion of selected clinical and pharmaceutical applications of this technique. Main application areas discussed will include (1) the use of SPME for ligand-receptor binding and plasma protein binding studies (2) the use of SPME to perform in vivo sampling of freely moving animals for pharmacokinetic studies (3) automation of SPME in 96-well plate format for high-throughput bioanalytical applications and (4) the use of SPME for direct brain and muscle tissue sampling. The discussion will include the comparison of the performance of SPME to various established methods relevant for particular application. The ability to use SPME to determine free and/or total drug and metabolite concentrations in biofluids will be emphasized because this is a unique advantage of SPME over traditional methods such as protein precipitation or solid-phase extraction. High-throughput SPME-LC-MS/MS applications such as the analysis of benzodiazepines in whole blood and the analysis of mycotoxins in urine will also be described and shown to meet regulatory guidelines for bioanalytical method validation. Finally, theoretical and practical aspects of in vivo SPME will be discussed as this promising new tool in bioanalysis provides not only effective sample clean-up prior to MS detection, but also eliminates the need to withdraw representative blood samples, thus facilitating single rodent pharmacokinetic studies.
Textbook for the course: Handbook of Solid Phase Microextraction, Pawliszyn, J. (editor).
Instructor:
Dr. Janusz Pawliszyn is the inventor of SPME and a professor in the department of Chemistry, University of Waterloo, Canada. He is the author of over 350 journal publications, 20 book chapters, and over 250 invited/plenary lectures. He has received several prestigious awards for his scientific achievements, and SPME is considered one of the six “Great Ideas of a Decade” by ACS Analytical Chemistry journal. He presently holds the Canada Research Chair and NSERC Industrial Research Chair in New Analytical Methods and Technologies. Secondary instructor: Dajana Vuckovic (Hon.B.Sc., University of Toronto, Canada, 2002) has three years of analytical method development and validation experience in pharmaceutical industry. Currently, she is Ph.D candidate in professor Pawliszyn’s group and the focus of her research is the development of SPME methodology for metabolomics, in vivo sampling, high-throughput bioanalysis as well as the development of new SPME coatings.
Short Course 4
MS of peptides and proteins
Instructor:
Prof Arthur Moseley
Duke University, USA
This is a full day course. The course fee includes a light lunch, two refreshment breaks and a copy of the course notes. The timings of the day will be confirmed in due course.
Cost: US$300
Summary will be added shortly
Instructor:
Arthur Moseley
Duke University, USA
